关键词] 免疫荧光;双重标记;激光共聚焦显微镜 [gap=1303]Key words] Immunofluorescence ; Double staining ; Laser confocal microscope ...
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The ovary tissues, ovarian surface peritoneum and vascular system architecture were observed under light microscope (LM). The distribution characteristic and species differences of ovarian vessel and lymphatic vessels were further clarify with 5’-nucleotidase-alkalinephosphatase (5’-nase-akpase) double staining.2.
经液氮冷冻作冰冻切片,行5’-核苷酸酶(5’-Nase)和碱性磷酸酶(ALP)双重染色,相邻组织切片做HE染色,光镜对照性观察卵巢组织和表面腹膜的细微结构,以及脉管系的构筑特点,进一步明确卵巢血管和淋巴管的分布特点及种属差异。
参考来源 - 卵巢表面结构特点和内部脉管系分布的形态学观察The tinction and morphology of MGC-803 cells treated with NGF at different final concentrations of 25,50 and 75mg/ L for 24h were observed by acridine orange/ethidium bromide (AO/EB) double staining.
采用AO/EB双重染色法,NGF浓度分别为25,50,75mg/L,观察不同浓度NGF作用后MGC-803细胞的染色及形态变化。
参考来源 - 广西眼镜蛇毒神经生长因子的亲和层析分离及其抗肿瘤作用·2,447,543篇论文数据，部分数据来源于NoteExpress
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